Composite

Part:BBa_K4160015:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


GEMS receptor construct containing HA-tag as affinity domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 97
    Illegal XbaI site found at 1727
    Illegal PstI site found at 415
    Illegal PstI site found at 1410
    Illegal PstI site found at 1571
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 97
    Illegal NheI site found at 784
    Illegal PstI site found at 415
    Illegal PstI site found at 1410
    Illegal PstI site found at 1571
    Illegal NotI site found at 1714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 97
    Illegal BglII site found at 888
    Illegal BglII site found at 1074
    Illegal BglII site found at 1338
    Illegal BamHI site found at 64
    Illegal XhoI site found at 298
    Illegal XhoI site found at 1721
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 97
    Illegal XbaI site found at 1727
    Illegal PstI site found at 415
    Illegal PstI site found at 1410
    Illegal PstI site found at 1571
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 97
    Illegal XbaI site found at 1727
    Illegal PstI site found at 415
    Illegal PstI site found at 1410
    Illegal PstI site found at 1571
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002, BBa_K1150016, and BBa_K2217015.

References